Journal: Virology journal
Article Title: Evaluation of ALKBH2 and ALKBH3 gene regulation in patients with adult T-cell leukemia/lymphoma.
doi: 10.1186/s12985-024-02590-w
Figure Lengend Snippet: Fig. 5 Investigation of DNA methylation states around the TSS of ALKBH3 in patients infected with HTLV-1. a–c CpG methylation states of T-cell subsets derived from HTLV-1-infected patients were investigated using a public dataset (GSE136189). Each CpG site was designated by a unique CpG cluster ID identifying the unique locus and by the number of nucleotide bases from the TSS of ALKBH3. Data are shown by β-value, which ranged from 0 to 1, denoting complete unmethylation and methylation, respectively. Scatter plots represent the median values. Significance was analyzed by one-way ANOVA followed by Tukey’s multiple comparisons test and is indicated by an asterisk. Statistical significance was observed in the analysis of cg14908875 (P vs. D: *p = 0.0272, P vs. N: ****p < 0.0001, P vs. Acu-N: ***p = 0.0004), cg21125143 (P vs. D: **p = 0.0069, P vs. N: ****p < 0.0001), cg26579032 (P vs. D: **p = 0.0038, P vs. N: ****p < 0.0001, N vs. Acu-N: *p = 0.0468), and cg26985203 (P vs. D: ****p < 0.0001, P vs. N: ****p < 0.0001, P vs. Acu-N: ****p < 0.0001, D vs. N: *p = 0.0390, D vs. Acu-N: *p = 0.0154). P, CD3+CADM1−CD7+ population; D, CD3+CADM1+CD7dim population; N, CD3+CADM1+CD7− population from chronic- and smoldering-type ATL; Acu-N, CD3+CADM1+CD7− population from acute-type ATL
Article Snippet: After washing with PBS containing 0.05% Tween 20 (PBS-T), the blocked membrane was incubated with rabbit anti-ALKBH2 monoclonal antibody (clone: EPR6177(2), Abcam, Cambridge, UK), rabbit anti-ALKBH3 polyclonal antibody (clone: 12292-1-AP, Proteintech Group, Inc., Rosemont, IL) or rabbit anti-beta actin polyclonal antibody (clone: 20536- 1-AP, Proteintech Group) in antibody dilution buffer (PBS-T containing 5% blocking solution) for 1 h at room temperature with shaking.
Techniques: DNA Methylation Assay, Infection, CpG Methylation Assay, Derivative Assay, Methylation